Biobanco del Instituto de Investigación del Hospital Universitario La Paz_IdiPAZ
The Hospital La Paz Institute for Health Research Biobank (IdiPAZ Biobank) is a a not-for-profit service platform that offers support to scientific community and promotes biomedical research. It originated as a product of the integration of different pre-existing nodes with a long and fruitful research trajectories within the University Hospital La Paz. IdiPAZ Biobank works to obtain, store, manage and distribute large collections of human biological samples and associated clinical data to scientific researchers ensuring protection of donor´s rights.
In 2020, the Biobank increases its capacity with the incorporatiof of new scientific-technical services, and as a result the platform has been renamed as Platform for Biobanks and Biomodels of IdiPAZ.
• Biobank HUB
• Organoid models HUB
• Tissue Engineering and 3D Printing Platform (PITI3D)
1. Biobank HUB
The main objective of this HUB is to maintain the identity of the Biobank as it has been operating over a decade, strengthening its structure and available resources as well as the management and coordination between IdiPAZ, HULP and external researchers.
The biobank has established circuits with the hospital services, in such a way that it has access to samples from the different clinical services. Likewise, the biobank has established lines of collaboration with other Institute platforms such as the Clinical Trials Research Unit (UICEC), the Innovation Support Platform (UAI) and the Internationalization Platform. In this way, the initiatives that arise in some of the platforms are mapped and collaborative synergies can be established in the different projects.
Biobank is estructure in four nodes:
• Tumor bank node: The IdiPAZ Biobank has available a catalog of the “Tumor Bank” collection at the service of the scientific community, with all types of samples obtained from solid tissue with different pathologies and tumours, both tumor and non-tumor. They are samples preserved both in OCT and embedded in paraffin.
• Biobank-children node: a strong effort has been made to promote the collection of samples in the pediatric population (Biobank Children), with established circuits in pediatric Neonatology and Hemato-oncology, obtaining a bank of sarcomas, lymphomas, leukemias, brain tumors, etc. This strategy has made it possible to increase the number of samples from the node as well as to establish genetic diagnoses and follow-up of patients.
• COVID node: given the 2020 health situation, a COVID-19 sample node has been established with surplus samples from the Hospital’s Clinical Services.
• Periferic laboratorics node: the biobank relies on the constant activity and synergy of several laboratories belonging to the HULP Health Research Institute (IdiPAZ) that actively collaborate with the hospital’s clinical services, in such a way that they collect samples dedicated to research projects but obtained with the Biobank’s Informed Consent, these samples can also be offered to the scientific community.
2. Organoid models HUB
IdiPaz´s Biobank is working on the stablishment of a living biobank of organoid models directly generated from patient biopsies. The in vitro organoid system is a critical tool for the study of a wide range of physiology processes and for drug screening studies to provide personalized treatment options to patients. Currently, we are generating a collection of living colonic organoids from both tumoral an peritumoral tissue from patients with colorectal cancer.
Areas of application:
• Studies of tissue morphogenesis and organ development
• Oncology and immuno-oncology
• Infectious diseases
• Genetic and inflammatory diseases
• Drug screening for toxicity and efficacy
3. Tissue Engineering and 3D Printing Platform (PITI3D)
The Tissue Engineering and 3D Printing Platform purpose is to promote the development of research and solutions based on tissue engineering and bioprinting and offer different services as computer imaging, virtual planning, computer aided design, and 3D printing technologies to the professionals of HULP and IDIPAZ.
The main areas of activities of the platform are:
• Bioprinting of tissues and organs
• Lab on a Chip (LOC) and Organ on a Chip (OOC) devices
• Tissue engineering approaches to develop tissue models and organs
• Development of new medical devices (biomodels, surgical guides, prosthesis and implants) based on emerging technologies as 3D printing
• Computer imaging, virtual planning and 3D printing to plan surgeries
Services
Technical and scientific evaluation
• Collection and management of the new collection
• Work Methodology/Methods
• Quality control of DNA/RNA sample treatment
• Training of experts to use the Biobank management system
• Training and advice on the use of Biobank management software (Bio-e-Bank).
• Information to the researcher about the custody, deposit, availability and transfer of the samples associated with the biobank.
• Training of practitioners (FCTs) of training cycles.
Sample/collection management:
• Coordinate a collection of samples at multiple study/research centers
• Storage of pre-existing collections according to the Biomedical Research Law (LIB 14/2007)
• Storage of pre-existing samples in accordance with the Spanish Biobank laws LIB 14/2007 and Royal Decree 1716/2011
• Management and control of Informed Consents (IC) and/or clinics regarding samples
• Aliquot of blood/saline solutions/plasma/other fluids
• Reception and coding of samples
Histology techniques:
• Manufacture of paraffin blocks
• Manufacture of frozen tissue blocks
• Paraffin/frozen sections
• Hematoxylin and eosin (H&E) stain
• Other types of staining
• Laser microdissection
Cellular techniques:
• Purification and freezing of peripheral blood mononuclear cells (PMBCs)
Molecular Techniques:
• Quantification of DNA and RNA using a spectrophotometer (NANODROP)
